The Effect of Different Endurance Exercise Intensities on the Expression of RIP140 Protein in Visceral Adipose Tissue in Diabetic Rats

Objective: RIP140 is one of the proteins that play a role in regulating and metabolizing lipid droplets. The aim of this study was to compare the three endurance training intensities (low, moderate and high) on the expression of RIP140 protein in visceral adipose tissue and insulin resistance in male diabetic wistar rats. Materials and Methods: Forty male wistar rats were assigned to five groups (n= 8) including diabetic group with low intensity endurance training (DLE), moderate intensity endurance training (DME), high intensity endurance training (DHE), diabetic control (DC) and healthy nondiabetic sedentary (NS) groups. After induction of diabetic rats by injection of streptozotocin, endurance training was performed with different intensities for eight weeks, three sessions per week. The relative expression of RIP140 protein was measured by western blot technique. To compare groups, dependent variables were analyzed by One Way ANOVA، Kruskal–Wallis and the post hoc Mann-Whitney U Test. Results: Results showed significant effect of exercise on insulin resistance values (P-value: 0.001). Comparison of expression of RIP140 protein in diabetic and high intensity endurance training group, diabetic and moderate intensity endurance training group and diabetic, and low intensity endurance training group, diabetic control and healthy control by using Kruskal-Wallis test indicated a significant difference between these groups (P-value:0.006). Conclusion: Moderate and high intensity endurance training can significantly reduce the expression of RIP140 protein, and subsequently increase glucose uptake and ultimately reduce insulin resistance in diabetic rats.